Modifying Enzymes
New England Biolabs, Inc. MseI – 2500 units
A restriction endonuclease that recognizes the sequence T^TA_A.
New England Biolabs, Inc. SP6 RNA Polymerase – 2000 units
Bacteriophage SP6 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the SP6 phage promoter. The 98.5 KD polymerase catalyzes in vitro RNA synthesis from a cloned DNA template under the SP6 promoter. RNA synthesized using the SP6 RNA Polymerase is suitable for many applications in research and biotechnology. Reaction Conditions: 1X RNAPol Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP and DNA template containing the SP6 RNA Polymerase promoter. Incubate at 37°C.
New England Biolabs, Inc. Bst DNA Polymerase, Large Fragment – 8000 units
Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5' to 3' polymerase activity, but lacks 5' to 3' exonuclease activity.
EPICYPHER INC CUTANA pAG-Tn5 for ChIC/CUT&Tag, 50 rxns
Recombinantly produced in E. coli, CUTANA pAG-Tn5 for ChIC/CUT&Tag is a fusion of Proteins A and G to Transposase Tn5. This construct is useful in performing Cleavage Under Targets and Tagmentation (CUT&Tag). The active dimer of Transposase Tn5 is charged with Illumina adapters and ready to be used immediately in CUT&Tag. CUTANA pAG-Tn5 does not contain an epitope tag, which makes it compatible with tag-mediated CUT&Tag.
New England Biolabs, Inc. USER® Enzyme – 50 units
USER (Uracil-Specific Excision Reagent) Enzyme generates a single nucleotide gap at the location of a uracil. USER Enzyme is a mixture of Uracil DNA glycosylase (UDG) and the DNA glycosylase-lyase Endonuclease VIII. UDG catalyses the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease VIII breaks the phosphodiester backbone at the 3 and 5 sides of the abasic site so that base-free deoxyribose is released.Applications of USER include:
- Directional RNA Seq
- NEBNext adaptor cleavage
- USER cloning
New England Biolabs, Inc. Mung Bean Nuclease – 1500 units
Mung Bean Nuclease is a single-strand specific DNA and RNA endonuclease which will degrade single-stranded extensions leaving blunt, ligatable ends.
Worthington Biochemical Corporation Deoxyribonuclease I, 25mg
Partially purified. A lyophilized powder. Store at 2-8°C. Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Worthington Biochemical Corporation PEROXIDASE HORSERADISH 100MG
A soluble, dialyzed, lyophilized powder. RZ > or = 1.0.
Worthington Biochemical Corporation COLLAGNASE 250MG (5 PK 50MG)
Collagenase, Type 3, Filtered, which is filtered through a 0.22 Micron membrane and lyophilized in vials to contain > /=50mg/VL, Store at 2 to 8degreeC, Pack Size: 5 x 50mg, Source: Clostridium histolyticum, Minimum Activity: > /=100U/mg dr
INTACT GENOMICS INC T4 DNA Ligase 100,000 Units (400 units/µl)
Intact Genomics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. This enzyme joins DNA fragments with either cohesive or blunt termini and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids (1).Applications• Cloning of restriction enzyme generated DNA fragments• Cloning of PCR products• Next-gen library preparation• Joining linkers and adapters to cohesive or blunt-ended DNA• Nick repair in duplex DNA, RNA or DNA/RNA hybrids• Self-circularization of linear DNA
Enzo Life Sciences Ubiquitin activating enzyme E1 (human), (recombinant) (His-tag) (50 µg)
Essential ubiquitinylation pathway enzyme. MW: 118 kDa. Purity: ≥90% (SDS-PAGE). Formulation: In 50mM TRIS-hydrochloric acid, containing 1.0mM dithiothreitol. Source: Produced in E. coli BL21 (λDE3) expression system and containing a C-terminal His6-tag. Full length human ubiquitin-activating enzyme E1. UniProt: P22314. Handling: Avoid freeze/thaw cycles. Long Term Storage: -80°C
Key Scientific Products DROP-IT CATALASE 3%, 5 per Pack
A Catalase test is useful for the identification of many different bacteria. Since Streptococci lack the enzyme to catalyze hydrogen peroxide, and all Staphylococci possess it, 3% Catalase is used to separate the species. Also, Listeria monocytogenes, which is catalase positive can be differentiated from beta-hemolytic Streptococci. Most Neisseria species are catalase positive. 15% Catalase is used for anaerobes, with most anaerobes being catalase negative with the exceptions of several species in the B. fragilis group and a few of the anaerobic cocci. 30% Catalase is used with acid fast bacilli.